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bio rad chemidoctm xrs system  (Bio-Rad)


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    Bio-Rad bio rad chemidoctm xrs system
    Bio Rad Chemidoctm Xrs System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 11421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bio+rad+chemidoctm+xrs+system/ChemiDoc+MP+System/pmc13094573-196-12-16
    Average 99 stars, based on 11421 article reviews
    bio rad chemidoctm xrs system - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Saline:

    Article Title: Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment.
    Article Snippet: After blocking with 5% milk powder at room temperature for 2 h, the membranes were incubated with primary antibodies against aggrecan (1:1000; 13880-1- AP; Proteintech, USA), iNOS (1:1000; ab178945; Abcam, USA) and β-actin (1:1000; A1978-10; Sigma-Aldrich, USA) at 4 oC overnight. .. Subsequently, the membranes were rinsed with phosphate-buffered saline with Tween® detergent (PBST) for three times and then incubated with mouse anti-rabbit IgG conjugated by horseradish peroxidase (Cell Signaling Technology, USA) at room temperature for 1 h. After rinsing, the PVDF membrane containing the blots and antibodies was placed in the Bio-Rad ChemiDocTM XRS system, and the Immobilon Western Chemiluminescent HRP Substrate (Millipore, USA) was applied to cover the membrane surface. .. Signal detection was captured using Image LabTM Software (Bio-Rad, China) and the intensity of the bands was quantified using Image J software.

    Article Title: Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment
    Article Snippet: After blocking with 5% milk powder at room temperature for 2 h, the membranes were incubated with primary antibodies against aggrecan (1:1000; 13880-1-AP; Proteintech, USA), iNOS (1:1000; ab178945; Abcam, USA) and β-actin (1:1000; A1978-10; Sigma-Aldrich, USA) at 4 oC overnight. .. Subsequently, the membranes were rinsed with phosphate-buffered saline with Tween ® detergent (PBST) for three times and then incubated with mouse anti-rabbit IgG conjugated by horseradish peroxidase (Cell Signaling Technology, USA) at room temperature for 1 h. After rinsing, the PVDF membrane containing the blots and antibodies was placed in the Bio-Rad ChemiDocTM XRS system, and the Immobilon Western Chemiluminescent HRP Substrate (Millipore, USA) was applied to cover the membrane surface. .. Signal detection was captured using Image LabTM Software (Bio-Rad, China) and the intensity of the bands was quantified using Image J software.

    Incubation:

    Article Title: Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment.
    Article Snippet: After blocking with 5% milk powder at room temperature for 2 h, the membranes were incubated with primary antibodies against aggrecan (1:1000; 13880-1- AP; Proteintech, USA), iNOS (1:1000; ab178945; Abcam, USA) and β-actin (1:1000; A1978-10; Sigma-Aldrich, USA) at 4 oC overnight. .. Subsequently, the membranes were rinsed with phosphate-buffered saline with Tween® detergent (PBST) for three times and then incubated with mouse anti-rabbit IgG conjugated by horseradish peroxidase (Cell Signaling Technology, USA) at room temperature for 1 h. After rinsing, the PVDF membrane containing the blots and antibodies was placed in the Bio-Rad ChemiDocTM XRS system, and the Immobilon Western Chemiluminescent HRP Substrate (Millipore, USA) was applied to cover the membrane surface. .. Signal detection was captured using Image LabTM Software (Bio-Rad, China) and the intensity of the bands was quantified using Image J software.

    Article Title: Butyrate Alleviates High-Fat-Induced Metabolic Disorders Partially through Increasing Systematic Glutamine.
    Article Snippet: Obesity has emerged as a worldwide epidemic.. Both butyrate and glutamine counteract obesity-related metabolic disorders; however, whether and how they synergistically cooperate with each other remains a mystery.. In the study, a high-fat diet (HFD, 60% calories from fat) was used to develop a model of obesity-related metabolic disorder and compared with administrated saline and sodium butyrate (SB, 300 mg/kg body weight) daily by gavage.

    Article Title: BOP1 contributes to the activation of autophagy in polycystic ovary syndrome via nucleolar stress response
    Article Snippet: The membranes were blocked with 5% dried skim milk for 1 h and then incubated with primary antibodies: BOP1[(Abcam, Rabbit, ab252819, 1:5000,used for human tissue and KGN cells), (Bioss, Rabbit, ab252819,1:1000,used for tissue of mice and mouse)], LC3B (Abcam, Rabbit, ab192890, 1:2000), SQSTM1 (Proteintech, Rabbit, 18420-1-AP 1:5000), Beclin1 (Proteintech, Rabbit, 11306-1-AP, 1:1000), RPL11 (Abcam, Rabbit, ab79352,1:1000), MDM2 (Santa Cruz, mouse, sc-965, 1:500), p53 (Proteintech, Rabbit, 60283-2-Ig, 1:5000), mTOR(Cell Signaling, Rabbit, 2972, 1:1000), p-mTOR (Cell Signaling, Rabbit, 2971, 1:1000), ERK(Proteintech, Rabbit, 11-257-1-AP), p-ERK (Cell Signaling, Rabbit, 4370, 1:2000), AMPK (Abcam, Rabbit, ab133448, 1:1000), p-AMPK (Cell Signaling, Rabbit, ab80039, 1:1000), AKT (Proteintech, mouse, 60203-2-Ig 1:1000), p-AKT (Proteintech, Rabbit, 80455-1-RR, 1:2000), and β-actin (Proteintech, Rabbit, 81115-1-RR 1:5000). .. The membrane was incubated with the primary antibodies for overnight at 4 °C and then rinsed thrice with TBST before treating with HRP conjugated Rabbit Anti-Goat IgG (H + L) or HRP conjugated Goat Anti-Mouse IgG (H + L) for 1 h. The bands were visualized using the Bio-Rad ChemiDocTM XRS + System. .. Proteins were extracted using the KGN cell lysis buffer and subjected to western blotting (Beyotime, Shanghai, China).

    Article Title: MicroRNA-582-3p regulates osteoporosis through regulating homeobox A10 and osteoblast differentiation.
    Article Snippet: The Western blot system was established using a Bio-Rad Bis–Tris Gel system following the manufacturer’s instructions. .. After the protein was transferred into polyvinylidene difluoride (PVDF) membrane, primary antibodies of anti-HOXA10 (26497-1-AP) and anti-GAPDH (ab8245) were prepared in 5% blocking buffer at a dilution of 1:1,000 and were incubated with the membrane at 4 C overnight, followed by washing and further incubation with goat anti-rabbit secondary antibody (ab190492, 1mg/ml, 1:10,000, Abcam Cambridge, UK) marked by horseradish peroxidase at room temperature for 1 h. After rinsing, the PVDF membrane carried blots and antibodies were transferred into the Bio-Rad ChemiDocTM XRS system, and then 200 ll Immobilon Western Chemiluminescent HRP Substrate (Millipore, MA) was added to cover the membrane surface. .. The signals were acquired and the intensity of the bands was quantified using Image LabTM Software (Bio-Rad, Shanghai, China) [26].

    Article Title: Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment
    Article Snippet: After blocking with 5% milk powder at room temperature for 2 h, the membranes were incubated with primary antibodies against aggrecan (1:1000; 13880-1-AP; Proteintech, USA), iNOS (1:1000; ab178945; Abcam, USA) and β-actin (1:1000; A1978-10; Sigma-Aldrich, USA) at 4 oC overnight. .. Subsequently, the membranes were rinsed with phosphate-buffered saline with Tween ® detergent (PBST) for three times and then incubated with mouse anti-rabbit IgG conjugated by horseradish peroxidase (Cell Signaling Technology, USA) at room temperature for 1 h. After rinsing, the PVDF membrane containing the blots and antibodies was placed in the Bio-Rad ChemiDocTM XRS system, and the Immobilon Western Chemiluminescent HRP Substrate (Millipore, USA) was applied to cover the membrane surface. .. Signal detection was captured using Image LabTM Software (Bio-Rad, China) and the intensity of the bands was quantified using Image J software.

    Membrane:

    Article Title: Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment.
    Article Snippet: After blocking with 5% milk powder at room temperature for 2 h, the membranes were incubated with primary antibodies against aggrecan (1:1000; 13880-1- AP; Proteintech, USA), iNOS (1:1000; ab178945; Abcam, USA) and β-actin (1:1000; A1978-10; Sigma-Aldrich, USA) at 4 oC overnight. .. Subsequently, the membranes were rinsed with phosphate-buffered saline with Tween® detergent (PBST) for three times and then incubated with mouse anti-rabbit IgG conjugated by horseradish peroxidase (Cell Signaling Technology, USA) at room temperature for 1 h. After rinsing, the PVDF membrane containing the blots and antibodies was placed in the Bio-Rad ChemiDocTM XRS system, and the Immobilon Western Chemiluminescent HRP Substrate (Millipore, USA) was applied to cover the membrane surface. .. Signal detection was captured using Image LabTM Software (Bio-Rad, China) and the intensity of the bands was quantified using Image J software.

    Article Title: BOP1 contributes to the activation of autophagy in polycystic ovary syndrome via nucleolar stress response
    Article Snippet: The membranes were blocked with 5% dried skim milk for 1 h and then incubated with primary antibodies: BOP1[(Abcam, Rabbit, ab252819, 1:5000,used for human tissue and KGN cells), (Bioss, Rabbit, ab252819,1:1000,used for tissue of mice and mouse)], LC3B (Abcam, Rabbit, ab192890, 1:2000), SQSTM1 (Proteintech, Rabbit, 18420-1-AP 1:5000), Beclin1 (Proteintech, Rabbit, 11306-1-AP, 1:1000), RPL11 (Abcam, Rabbit, ab79352,1:1000), MDM2 (Santa Cruz, mouse, sc-965, 1:500), p53 (Proteintech, Rabbit, 60283-2-Ig, 1:5000), mTOR(Cell Signaling, Rabbit, 2972, 1:1000), p-mTOR (Cell Signaling, Rabbit, 2971, 1:1000), ERK(Proteintech, Rabbit, 11-257-1-AP), p-ERK (Cell Signaling, Rabbit, 4370, 1:2000), AMPK (Abcam, Rabbit, ab133448, 1:1000), p-AMPK (Cell Signaling, Rabbit, ab80039, 1:1000), AKT (Proteintech, mouse, 60203-2-Ig 1:1000), p-AKT (Proteintech, Rabbit, 80455-1-RR, 1:2000), and β-actin (Proteintech, Rabbit, 81115-1-RR 1:5000). .. The membrane was incubated with the primary antibodies for overnight at 4 °C and then rinsed thrice with TBST before treating with HRP conjugated Rabbit Anti-Goat IgG (H + L) or HRP conjugated Goat Anti-Mouse IgG (H + L) for 1 h. The bands were visualized using the Bio-Rad ChemiDocTM XRS + System. .. Proteins were extracted using the KGN cell lysis buffer and subjected to western blotting (Beyotime, Shanghai, China).

    Article Title: MicroRNA-582-3p regulates osteoporosis through regulating homeobox A10 and osteoblast differentiation.
    Article Snippet: The Western blot system was established using a Bio-Rad Bis–Tris Gel system following the manufacturer’s instructions. .. After the protein was transferred into polyvinylidene difluoride (PVDF) membrane, primary antibodies of anti-HOXA10 (26497-1-AP) and anti-GAPDH (ab8245) were prepared in 5% blocking buffer at a dilution of 1:1,000 and were incubated with the membrane at 4 C overnight, followed by washing and further incubation with goat anti-rabbit secondary antibody (ab190492, 1mg/ml, 1:10,000, Abcam Cambridge, UK) marked by horseradish peroxidase at room temperature for 1 h. After rinsing, the PVDF membrane carried blots and antibodies were transferred into the Bio-Rad ChemiDocTM XRS system, and then 200 ll Immobilon Western Chemiluminescent HRP Substrate (Millipore, MA) was added to cover the membrane surface. .. The signals were acquired and the intensity of the bands was quantified using Image LabTM Software (Bio-Rad, Shanghai, China) [26].

    Article Title: Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment
    Article Snippet: After blocking with 5% milk powder at room temperature for 2 h, the membranes were incubated with primary antibodies against aggrecan (1:1000; 13880-1-AP; Proteintech, USA), iNOS (1:1000; ab178945; Abcam, USA) and β-actin (1:1000; A1978-10; Sigma-Aldrich, USA) at 4 oC overnight. .. Subsequently, the membranes were rinsed with phosphate-buffered saline with Tween ® detergent (PBST) for three times and then incubated with mouse anti-rabbit IgG conjugated by horseradish peroxidase (Cell Signaling Technology, USA) at room temperature for 1 h. After rinsing, the PVDF membrane containing the blots and antibodies was placed in the Bio-Rad ChemiDocTM XRS system, and the Immobilon Western Chemiluminescent HRP Substrate (Millipore, USA) was applied to cover the membrane surface. .. Signal detection was captured using Image LabTM Software (Bio-Rad, China) and the intensity of the bands was quantified using Image J software.

    Western Blot:

    Article Title: Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment.
    Article Snippet: After blocking with 5% milk powder at room temperature for 2 h, the membranes were incubated with primary antibodies against aggrecan (1:1000; 13880-1- AP; Proteintech, USA), iNOS (1:1000; ab178945; Abcam, USA) and β-actin (1:1000; A1978-10; Sigma-Aldrich, USA) at 4 oC overnight. .. Subsequently, the membranes were rinsed with phosphate-buffered saline with Tween® detergent (PBST) for three times and then incubated with mouse anti-rabbit IgG conjugated by horseradish peroxidase (Cell Signaling Technology, USA) at room temperature for 1 h. After rinsing, the PVDF membrane containing the blots and antibodies was placed in the Bio-Rad ChemiDocTM XRS system, and the Immobilon Western Chemiluminescent HRP Substrate (Millipore, USA) was applied to cover the membrane surface. .. Signal detection was captured using Image LabTM Software (Bio-Rad, China) and the intensity of the bands was quantified using Image J software.

    Article Title: MicroRNA-582-3p regulates osteoporosis through regulating homeobox A10 and osteoblast differentiation.
    Article Snippet: The Western blot system was established using a Bio-Rad Bis–Tris Gel system following the manufacturer’s instructions. .. After the protein was transferred into polyvinylidene difluoride (PVDF) membrane, primary antibodies of anti-HOXA10 (26497-1-AP) and anti-GAPDH (ab8245) were prepared in 5% blocking buffer at a dilution of 1:1,000 and were incubated with the membrane at 4 C overnight, followed by washing and further incubation with goat anti-rabbit secondary antibody (ab190492, 1mg/ml, 1:10,000, Abcam Cambridge, UK) marked by horseradish peroxidase at room temperature for 1 h. After rinsing, the PVDF membrane carried blots and antibodies were transferred into the Bio-Rad ChemiDocTM XRS system, and then 200 ll Immobilon Western Chemiluminescent HRP Substrate (Millipore, MA) was added to cover the membrane surface. .. The signals were acquired and the intensity of the bands was quantified using Image LabTM Software (Bio-Rad, Shanghai, China) [26].

    Article Title: Unveiling the therapeutic potential of phenolic compounds from Boletus edulis in osteoarthritis treatment
    Article Snippet: After blocking with 5% milk powder at room temperature for 2 h, the membranes were incubated with primary antibodies against aggrecan (1:1000; 13880-1-AP; Proteintech, USA), iNOS (1:1000; ab178945; Abcam, USA) and β-actin (1:1000; A1978-10; Sigma-Aldrich, USA) at 4 oC overnight. .. Subsequently, the membranes were rinsed with phosphate-buffered saline with Tween ® detergent (PBST) for three times and then incubated with mouse anti-rabbit IgG conjugated by horseradish peroxidase (Cell Signaling Technology, USA) at room temperature for 1 h. After rinsing, the PVDF membrane containing the blots and antibodies was placed in the Bio-Rad ChemiDocTM XRS system, and the Immobilon Western Chemiluminescent HRP Substrate (Millipore, USA) was applied to cover the membrane surface. .. Signal detection was captured using Image LabTM Software (Bio-Rad, China) and the intensity of the bands was quantified using Image J software.

    Imaging:

    Article Title: Gelatin methacryloyl microneedle loaded with 3D-MSC-Exosomes for the protection of ischemia-reperfusion.
    Article Snippet: Exosomes (Exo) generated from mesenchymal stem cells (MSCs) have great therapeutic potential in ischemiareperfusion treatment.. For best therapeutic effect, high quality Exo product and effective delivery system are indispensable.. In this study, we developed a new strategy for ischemia-reperfusion recovery by combining MSCs 3D (3D-MSC) culturing technology to generate Exo (3D-MSC-Exo) and microneedle for topical delivery.

    Blocking Assay:

    Article Title: MicroRNA-582-3p regulates osteoporosis through regulating homeobox A10 and osteoblast differentiation.
    Article Snippet: The Western blot system was established using a Bio-Rad Bis–Tris Gel system following the manufacturer’s instructions. .. After the protein was transferred into polyvinylidene difluoride (PVDF) membrane, primary antibodies of anti-HOXA10 (26497-1-AP) and anti-GAPDH (ab8245) were prepared in 5% blocking buffer at a dilution of 1:1,000 and were incubated with the membrane at 4 C overnight, followed by washing and further incubation with goat anti-rabbit secondary antibody (ab190492, 1mg/ml, 1:10,000, Abcam Cambridge, UK) marked by horseradish peroxidase at room temperature for 1 h. After rinsing, the PVDF membrane carried blots and antibodies were transferred into the Bio-Rad ChemiDocTM XRS system, and then 200 ll Immobilon Western Chemiluminescent HRP Substrate (Millipore, MA) was added to cover the membrane surface. .. The signals were acquired and the intensity of the bands was quantified using Image LabTM Software (Bio-Rad, Shanghai, China) [26].



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